Estimation of Probe Cell Locations in High-density Synthetic-oligonucleotide DNA Microarrays
نویسندگان
چکیده
The source of a spatial contribution to the coefficient of variation in Affymetrix GeneChip r © probe cell summaries was determined to be caused by a misalignment of probe cell locations in the hybridisation image. Described, is an algorithm that corrects for misalignment resulting in improved allocation of pixel intensities to probe cells and a substantial reduction in the variance of pixel intensities attributed to individual probe cells. Institute of Statistics and Decision Sciences, Duke University, Durham, NC 27708 Departments of Surgery and Genetics, Duke University Medical Center, Durham, NC 27710 Howard Hughes Medical Institute, Durham, NC 27710
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